Project Summaries

12-191  Project Manager: D. C. Jones


David Fang, USDA-ARS

The primary objective is to improve fiber strength using conventional and molecular breeding techniques and eventually breed cotton germplasm with high fiber quality.  The specific objectives of this project are: 1) understanding the genetics of the enhanced fiber strength contributed by fiberless diploid D genome species G. armourianum; 2) determining the genomic regions harboring the enhanced fiber strength; and 3)developing a population suitable for mapping, and laying the foundation to assist the introgression of this trait into cotton varieties with molecular markers.

We identified more than 400 SSR markers that were specific to G. hirsutum cv SG747, G. arboreum, G. herbaceum, and G. armourianum.  We also developed BC2F2 and BC3F1 populations using SG747 as recurrent parent and collected fiber samples from about 250 BC2F1 plants in order to identify BC2F1 plants that are suitable for mapping population development.

We analyzed about 1000 SSR markers among G. hirsutum cv. SG747, G. arboreum, G. herbaceum, and G. armourianum. Almost all SSR markers analyzed were polymorphic among these four species. More than 100 species-specific marker alleles were identified for each species.  The introgression populations developed were backcrossed to SG747; however, the BC1F1 plants showed pollen sterility.  To recover fertility, 17 BC1F1 plants were backcrossed to SG747 to generate BC2F1 plants.  Seed from the crosses were planted in Stoneville, MS during 2012 to develop BC2F2 and BC3F1 populations. Cotton bolls were collected from each one of BC2F1 plants. However, due to prevalent sterility, some BC2F1 plants did not produce bolls or produced a few.  We collected bolls from 311 plants belonging to 14 BC2F1 families.  We are analyzing fiber quality (strength, length, micronaire, fineness etc.) using HVI.  Plants showing high fiber strength (as compared to SG747) will be an indication that the enhanced fiber strength trait is maintained. BC2F2 and BC3F1 seeds derived from these plants will be advanced next year.


Project Year: 2012

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