Project Summaries

12-188  Project Manager: D. C. Jones

GENETIC DISSECTION OF HETEROTIC EFFECTS IN UPLAND COTTON

David Fang, USDA-ARS

This is part of the research project entitled "Genetic Dissection of Heterotic Effects in Upland Cotton" that Todd Campbell is the principal investigator. In the present project, the specific objective is to analyze molecular markers among the 283 F2 progeny plants derived from a cross between Georgia King and FM966, construct an intraspecific map, and identify molecular markers associated with yield QTLs.

In order to identify polymorphic markers between two parents, we screened 2989 SSR and 308 SNP markers. Of them, 715 SSR (23.9%) and 3 SNP (0.97%) markers were polymorphic between parents. The high polymorphic rate for SSR markers was mainly due to the fact that all 2989 SSR markers were pre-screened using different panel, and known to be polymorphic within Gossypium hirsutum. The SNPs screened were from a publication (Byers et al. 2012) and most of the SNPs appeared monomorphic within Upland cotton.

In 2012, we analyzed 567 SSR markers among 283 F2 progeny. A preliminary analysis of these data indicated that the marker loci segregated as expected in an F2 population.  All SSR markers were genotyped using an ABI3730 Genetic Analyzer. Each marker data point needs to be extracted from the raw data files using GeneMapper software.  As of today, all 567 SSR markers were extracted and tabulated in a spread sheet.  An intraspecific map has not been constructed mainly because approximately 150 polymorphic markers were analyzed among F2 populations. We will construct a map after all polymorphic markers are analyzed.

 

Project Year: 2012
 

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