Project Summaries

12-118TX  Project Manager: D. C. Jones

SPECTROSCOPIC APPROACH TO STUDY COTTON FIBER MATURITY

Noureddine Abidi, Texas Tech University

The structure of cellulose in cotton fibers at varying developmental stages was investigated using Fourier Transform Infrared (FTIR) spectroscopy technique. Bolls from greenhouse-grown cottons cultivars (Texas Marker-1 and TX55 cultivars) were harvested between 10 and 56 dpa (days post anthesis). The fibers were dried at 40oC for 2 days and conditioned in a laboratory maintained at 21 ± 1oC and 65 ± 1% relative humidity for at least 2 days prior to FTIR analysis. The FTIR spectra were acquired directly on the bundle of fibers with no further preparation using Universal Attenuated Total Reflectance FTIR in the mid-IR. The analysis of the FTIR spectra revealed very important information regarding the structural development of cellulose in cotton fibers. The principal component analysis of the FTIR spectra revealed three clusters of spectra. Our results showed that the integrated intensities of the vibration located at 3286, 1738, 1639, 1543, 1161, 897, 710, and 667 cm-1 could be used to investigate the development of the secondary cell wall in cotton fibers. In addition, there was very good correlation between the integrated intensities of the vibrations located at 897 and 667 cm-1 and the cellulose content. These two vibrations could be used to estimate indirectly the cellulose content in cotton fibers.

 

Project Year: 2012
 

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