Project Summaries

11-897  Project Manager: D. C. Jones

USE OF CHROMOSOME SPECIFIC RECOMBINANT INBRED LINES (CSRIL) TO DEVELOP IMPROVED GERMPLASM AND TO DISCOVER NOVEL ALLELES FOR IMPROVED AGRONOMIC AND FIBER PROPERTIES IN UPLAND COTTON.

Sukumar Saha, USDA-ARS

The overall objective of this research was to evaluate genetic variations for fiber and agronomic traits of two chromosome-specific recombinant-inbred line (CSRIL) populations involving segments of chromosomes 22 short arm, chromosome 22 long arm, and chromosome 25 from Gossypium barbadense in G. hirsutum genetic background. The CSRILs were developed from crosses of CS-B22sh, CS-B22Lo and CS-B25 G. barbadense chromosome substitution lines with their common recurrent parent, inbred G. hirsutum TM-1. The population included 30 lines of CS-B22Lo, 39 lines of 22sh, 32 lines of CS-B25 RILs, and the respective parental lines which were planted on May 13 with commercial varieties DP393 and PHY 370 WR in two environments on the Plant Science Farm at Mississippi State, MS.  In each environment, the experimental design was a randomized complete block with four replications. Plots were a single row about 12 M long and rows were spaced 96.5 cm apart. Plants within the row were spaced 10 cm apart, so that there were about 120 plants in each plot. Yield was determined by harvesting with a two row mechanical cotton picker equipped with load cells for individual rows. Before machine harvest, a 25 boll sample was hand harvested from bolls near the center of the plant from individual CSRIL for measuring data on fiber traits at STARLAB Inc. in Knoxville, TN. We are currently in the process of analyzing the fiber data at Star Lab. The fiber traits recorded were length (Upper half mean UHM, mm), uniformity (%), strength (kN m kg-1), fiber elongation (%), micronaire, reflectance (Rd), and yellowness (b) using a High Volume Instruments (HVI). We also collected the leaf samples from individual CSRIL line for DNA extraction. Currently we are in the process of genotyping the individual lines with the polymorphic chromosome specific SSR markers for the respective chromosome. We plan to repeat the experiments in the next summer of 2013 to study GxE interaction effects. After the completion of 2013 experiments we will analyze the two year data from multiple locations to study the genetic effects using an additive-dominance (AD) model. Our preliminary observation from the experiments suggested that significant differences exist among the RI lines specific to a chromosome or chromosome segment.

 

Project Year: 2012
 

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