Project Summaries

10-797  Project Manager: D. C. Jones

DEVELOPMENT OF A DOUBLED HAPLOID INDUCER RESOURCE IN COTTON

Allen Van Deynze, University of California, Davis

The production of doubled haploid lines has revolutionized breeding programs in maize and canola. In cotton they would significantly shorten breeding cycles and would enable integration of genomics into breeding thru genome selection.  The rapid production of true-breeding populations would significantly aid in genome assembly and annotation by aligning the thousands of genomic assemblies (scaffolds) and assigning them to chromosomes.  Only a few recombinant inbred populations are publicly available currently.  They would also enable high-resolution genetic studies allowing for highly replicated phenotypic analysis.

A UC Discovery matching grant was acquired to extend funding for this research for 2011 and 2012. The project was initiated in September 2010 with the goal of developing/establishing a refined transformation system for cotton. The general goals are to develop and evaluate a general inducer line to produce doubled haploids in cotton species. We have successfully transformed Coker 312 with a CenH3 construct using a modified transformation system significantly reducing time for transformation. The long-term goals of this project are: 1) Develop gene constructs to simultaneously silence endogenous CENH3 and create a haploid inducer by expressing a modified GFP-CENH3, 2)Transform cotton with gene construct for CENH3, and 3) Characterize gene constructs molecularly and for capacity to induce haploid plants in several genetic cotton backgrounds.

In 2012 we achieved our first and second goals: to create CenH3 constructs and transform Coker312. The project timeline has been significantly extended for two reasons 1) the complexity of defining the CenH3 promoter without a genomic sequence and 2) the very poor efficiency of transformation. We have made significant progress and addressed both these limitations.

 

Project Year: 2012
 

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CORE PROGRAM
▸ Cotton Incorporated Fellow
▸ Cottonseed
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