Project Summaries

09-541  Project Manager: D. C. Jones

INTROGRESS GOSSYPIUM BARBEDENSE I. ALLELES INTO ELITE G. HIRSUTUM GERMPLASM VIA RANDOM MATING

Johnie N. Jenkins, USDA-ARS

We crossed 18 CS-B lines with FM966, SG747, and PSC 355.  The CS-B lines were CS-B 01, 02, 04, 06, 07, 10, 16, 17, 18, 25, 5sh, 11sh, 12sh, 14sh, 15sh, 22sh, 22Lo, and 26Lo.  F1s of these crosses were planted in Mexico in the winter nursery (2008-09) and the F1 plants were randomly intercrossed using bulked pollen.  This was designated Cycle 0.  After the first cycle of random mating, we pooled the three variety lines within each CS-B family into 18 half-sib families and kept the 18 individual populations as half sib families during the following cycles of random mating.  Random mating was among all 18 families.  We have now completed 5 cycles of random mating.  After the fifth cycle (C4) seed were self-pollinated in the winter nursery to provide seed for release.

In the fourth quarter we made an official germplasm release of RMBUP-C4 S1 seed and had a registration manuscript accepted for publication in Journal of Plant Registration with the required one pound of seed deposited in the National Germplasm Repository at Ft. Collins, Colorado. We made the first generation of self-pollinations for development of 25 RILs from each of the 18 half sib families and planted seed in the greenhouse for the second generation of self-pollination for the RIL lines.

 

Project Year: 2012
 

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